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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
20G2C12, monoclonal
分子量
calculated mol wt 21.59 kDa
observed mol wt ~25 kDa
物種活性
human
包裝
antibody small pack of 100 μL
技術
co-immunoprecipitation (co-IP): suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
UniProt登錄號
運輸包裝
dry ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... TFAM(7019)
一般說明
Transcription factor A, mitochondrial (UniProt: Q00059; also known as mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM) is encoded by the TFAM (also known as TCF6, TCF6L2) gene (Gene ID: 7019) in human. TFAM is a DNA binding protein that plays multiple roles in regulating mitochondrial DNA function. As a sequence-specific transcription factor, it binds upstream of the light strand promoter and heavy strand promoter 1 to activate initiation of transcription. It is a component of the mitochondrial transcription initiation complex (composed of TFB2M, TFAM and POLRMT). In this complex, TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. TFAM contains two HMG boxes (aa 50-118 and 155-219). Phosphorylation by protein kinase A within the HMG box 1 is shown to impair its DNA binding and promotes its degradation by AAA+ Lon protease. Mutations in TFAM gene are known to cause hepatocerebral type of mitochondrial DNA depletion syndrome that is characterized by severe intrauterine growth restriction, neonatal-onset hypoglycemia and liver dysfunction. In this disease hepatic pathology includes cirrhosis, steatosis and cholestasis that leads to liver failure. (Ref.: Wang, YE., et al. (2013). PLoS ONE. 8(8); e74513).
特異性
Clone 20G2C12 is a mouse monoclonal antibody that detects human mitochondrial Transcription Factor A (TFAM).
免疫原
Full-length recombinant human Transcription factor A, mitochondrial (TFAM).
應用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Tested Applications
ChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.
Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TFAM in HeLa cell lysates.
Tested Applications
ChIP-seq Analysis: A representative lot detected TFAM in ChIP-seq applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunoprecipitation Analysis: A representative lot immunoprecipitated TFAM in Immunoprecipitation applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TFAM in HeLa cells.
Western Blotting Analysis: A representative lot detected TFAM in Western Blotting applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Immunocytochemistry Analysis: A representative lot detected TFAM in Immunocytochemistry applications (Wang, Y.E., et. al. (2013). PLoS One. 8(8):e74513).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-TFAM, clone 20G2C12, Cat. No. MABE1994, is a mouse monoclonal antibody that detects Transcription factor A, mitochondrial (TFAM) and is tested for use in ChIP-seq, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at +2°C to +8°C from date of receipt.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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