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生物源
hamster
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
Mags-21F7, monoclonal
分子量
observed mol wt ~75 kDa
純化經由
using protein G
物種活性
human, mouse
包裝
antibody small pack of 100
技術
flow cytometry: suitable
western blot: suitable
同型
IgG2
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
human ... ZBTB16(7704)
特異性
Clone Mags-21F7 is an Armenian Hamster monoclonal antibody that detects Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
免疫原
A combination of peptides covering the amino, carboxyl, and hinge regions of human Zinc finger and BTB domain-containing protein 16 (PLZF/ZBTB16).
應用
Quality Control Testing
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant Human PLZF/ZBTB16 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, FLAG-tagged recombinant Human PLZF/ZBTB16.
Tested Applications
Western Blotting Analysis: A representative lot detected PLZF/ZBTB16 in Western Blotting applications (Kovalovsky, D., et al. (2008). Nat Immunol. 9(9):1055-64).
Flow Cytometry Analysis: A representative lot detected PLZF/ZBTB16 in Flow Cytometry applications (Kovalovsky, D., et al. (2008). Nat Immunol.;9(9):1055-64; Kovalovsky, D., et al. (2010). J Immunol. 184(12):6746-55).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Zinc finger and BTB domain-containing protein 16 (UniProt: Q05516; also known as Promyelocytic leukemia zinc finger protein, Zinc finger protein 145, Zinc finger protein PLZF) is encoded by the ZBTB16 (also known as PLZF, ZNF145) gene (Gene ID: 7704) in human. PLZF is a member of the krueppel C2H2-type zinc-finger protein family with nine C2H2-tpe zinc-finger domains. It serves as a transcriptional repressor and this repression may be mediated through recruitment of histone deacetylases to target promoters. It affects diverse signaling pathways, including growth-regulatory and differentiation pathways and is a key regulator of myeloid development. Its expression is observed in early, but in differentiated hematopoietic cells. It is expressed in bone marrow, early myeloid cells, and peripheral blood mononuclear cells. At lower level it is also detected in the ovary, kidney, and lung tissue. PLZF is reported to be essential for development of the characteristic innate/effector functions of natural killer T (NKT) cells. It is highly expressed during the differentiation of NKT cells and is essential for the acquisition of effector/memory innate-like functions. PLZF-deficient NKT cells lose their characteristic memory phenotype, and their function is significantly impaired. These cells lose their unique ability to co-secrete IL-4 and IFN- upon ex vivo stimulation. (Ref.: Kolesnichenko, M., and Vogt, PK. (2011). Cell Cycle.10(5); 771-775; Kovalovsky, D., et al. (2010). J. Immunol. 184: 6746-6755; Melnick, AM., et al. (2000). Mol. Cell Biol. 20(6); 2075-2086).
外觀
Purified Armenian hamster monoclonal antibody IgG2-IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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