MABE1803
Anti-SPT5 Antibody, clone 6F1
clone 6F1, from rat
别名:
Transcription elongation factor SPT5, hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DSIF p160, DRB sensitivity-inducing factor large subunit, DSIF large subunit, Tat-co-transactivator 1 protein, Tat-CT1 protein
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所有图片(2)
About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
6F1, monoclonal
物種活性
human
技術
ChIP: suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
human ... SUPT5H(6829)
一般說明
Transcription elongation factor SPT5 (UniProt: O00267; also known as hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DSIF p160, DRB sensitivity-inducing factor large subunit, DSIF large subunit, Tat-co-transactivator 1 protein, Tat-CT1 protein) is encoded by the SUPT5H (also known as SPT5, SPT5H) gene (Gene ID: 6829) in human. SPT5 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF is a heterodimer of SPT4 and SPT5 that positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing can facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II, which binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3′ terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. SPT5 can be methylated by PRMT1/HRMT1L2 and PRMT5/SKB1 and this methylation negatively regulates interaction with the positive transcription elongation factor (P-TEFb) and RNA polymerase II.
特異性
Clone 6F1 specifically detects SPT5 in HEK293T and Jurkat cells. It targets an epitope of 18 amino acids within the C-terminal half.
免疫原
Ovallbumin-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Transcription elongation factor SPT5.
應用
Anti-SPT5, clone 6F1, Cat. No. MABE1803, is a highly specific rat monoclonal antibody that targets Transcription elongation factor SPT5 and has been tested in Chromatin Immunoprecipitation (ChIP) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SPT5 in 10 µg of HEK293T cell lysate.
Chromatin Immunoprecipitation Analysis: A representative lot detected SPT5 in Chromatin Immunoprecipitation applications (Kelso, T.W., et. al. (2014). Mol Cell Biol. 34(19):3675-88).
Chromatin Immunoprecipitation Analysis: A representative lot detected SPT5 in Chromatin Immunoprecipitation applications (Kelso, T.W., et. al. (2014). Mol Cell Biol. 34(19):3675-88).
品質
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SPT5 in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected SPT5 in 10 µg of Jurkat cell lysate.
標靶描述
~121 kDa observed; 121 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
Danya J Martell et al.
Developmental cell, 58(20), 2112-2127 (2023-08-17)
Controlled release of promoter-proximal paused RNA polymerase II (RNA Pol II) is crucial for gene regulation. However, studying RNA Pol II pausing is challenging, as pause-release factors are almost all essential. In this study, we identified heterozygous loss-of-function mutations in
Felipe Beckedorff et al.
Cell reports, 32(3), 107917-107917 (2020-07-23)
Transcription by RNA polymerase II (RNAPII) is pervasive in the human genome. However, the mechanisms controlling transcription at promoters and enhancers remain enigmatic. Here, we demonstrate that Integrator subunit 11 (INTS11), the catalytic subunit of the Integrator complex, regulates transcription
Kaiwei Liang et al.
Cell, 175(3), 766-779 (2018-10-20)
The super elongation complex (SEC) is required for robust and productive transcription through release of RNA polymerase II (Pol II) with its P-TEFb module and promoting transcriptional processivity with its ELL2 subunit. Malfunction of SEC contributes to multiple human diseases
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