推荐产品
生物源
rat
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
103, monoclonal
物種活性
rat, mouse
技術
immunohistochemistry: suitable
western blot: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
mouse ... Nfe2L2(18024)
一般說明
Nuclear factor erythroid 2-related factor 2 (UniProt: Q60795; also known as Nrf2, NF-E2-related factor 2, NFE2-related factor 2, Nuclear factor, erythroid derived 2, like 2) is encoded by the Nfe2l2 (also known as Nrf-2, Nrf2) gene (Gene ID: 18024) in murine species. Nrf2 is a cap n collar basic-region leucine zipper transcription factor that is involved in adaptation of cells to oxidative stress and electrophiles. It bind to antioxidant response (ARE) elements in the promoter regions of target genes and induces a set of cytoprotective genes that are involved in the synthesis of reduced glutathione (GSH), repair of oxidized protein thiols, scavenging of reactive oxygen species, and drug metabolism. It is cytosolic under unstressed conditions, but translocates into the nucleus upon induction by electrophilic agents. Under normal unstressed conditions it is rapidly turned over in a Keap-1 dependent manner through Cul3-Rbx1 ubiquitination and proteasomal degradation. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates Nrf2 from its Keap-1 and promotes its translocation into the nucleus. It is widely expressed with highest expression in liver, skeletal muscle, luminal cells of the stomach and intestine, lining of the bronchi and alveoli, and in renal tubules; followed by heart, spleen, testis and brain. Nrf2 is constitutively upregulated in many tumors due to somatic mutations in the Keap1 or Nrf2 genes. Tumors overexpressing Nrf2 exhibit increased resistance to chemotherapeutic drugs and higher rates of proliferation. (Ref.: Hayes, JD., and Ashford, MLJ (2012). Cell Metab. 16(2): 139-141).
特異性
Clone 103 detects Nrf2 in Keap-1 knockout cells. It targets an epitope within 179 amino acids in the internal region.
免疫原
GST-tagged recombinant fragment corresponding to 179 amino acids from internal region of murine Nuclear factor erythroid 2-related factor 2.
應用
Anti-Nrf2, clone 103 , Cat. No. MABE1799, is a highly specific rat monoclonal antibody that targets Nuclear factor erythroid 2-related factor 2 and has been tested in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Nrf2 in Immunohistochemistry applications (Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84).
Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).
Western Blotting Analysis: A representative lot detected Nrf2 in Western Blotting applications (Maruyama, A., et. al. (2008). Arch Biochem Biophys. 477(1):139-45; Kobayashi, E.H., et. al. (2016). Nat Commun. 7:11624; Taquchi, K., et. al. (2016). Toxicol Sci. 152(1):40-52; Saito, R., et. al. (2015). Mol Cell Biol. 36(2):271-84; Taquchi, K., et. al. (2014). Mol Cell Biol. 34(5):900-13).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
品質
Evaluated by Western Blotting in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nrf2 in Keap1-KO MEF cell lysates versus Nrf2-KO MEF cell lysates.
標靶描述
~67 kDa observed; 66.90 kDa calculated Uncharacterized bands may be observed in some lysate(s).
外觀
Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
Ye Tian et al.
PloS one, 18(1), e0279964-e0279964 (2023-01-07)
Sepsis associated encephalopathy (SAE) is a common but poorly understood complication during sepsis. Currently, there are no preventive or therapeutic agents available for this neurological disorder. The present study was designed to determine the potential protective effects of β-patchoulene (β-PAE)
Carmen Rodríguez-García et al.
Molecular nutrition & food research, 66(18), e2200082-e2200082 (2022-07-19)
To compare the effects of three high-fat diets (HFDs) based on coconut, sunflower, or extra virgin olive oils (EVOOs) on adipose tissue, metabolism, and inflammation. Mice are fed for 16 weeks on their respective HFD. HFD based on coconut oil produces
Yuening Liu et al.
The Journal of biological chemistry, 297(1), 100850-100850 (2021-06-05)
Reperfusion therapy, the standard treatment for acute myocardial infarction, can trigger necrotic death of cardiomyocytes and provoke ischemia/reperfusion (I/R) injury. However, signaling pathways that regulate cardiomyocyte necrosis remain largely unknown. Our recent genome-wide RNAi screen has identified a potential necrosis
Sirinat Pengnet et al.
Frontiers in pharmacology, 13, 1049818-1049818 (2023-01-03)
Background: Excessive fructose consumption causes hepatic lipid accumulation via increased triglyceride (TG) synthesis, leading to the development and progression of non-alcoholic fatty liver disease (NALFD). Naringin, a flavanone glycoside found in citrus fruit, has antioxidant and hypolipidemic properties. Therefore, the
Greg A Timblin et al.
Nature metabolism, 3(5), 618-635 (2021-05-26)
Macrophages generate mitochondrial reactive oxygen species and mitochondrial reactive electrophilic species as antimicrobials during Toll-like receptor (TLR)-dependent inflammatory responses. Whether mitochondrial stress caused by these molecules impacts macrophage function is unknown. Here, we demonstrate that both pharmacologically driven and lipopolysaccharide
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门