生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3Ti-3C11, monoclonal
分子量
calculated mol wt 159 kDa
observed mol wt ~85 kDa
純化經由
using protein G
物種活性
mouse, human
物種活性（以同源性預測）
feline, horse
包裝
antibody small pack of 100
技術
ChIP: suitable
ELISA: suitable
western blot: suitable
同型
IgG1κ
表位序列
N-terminal half
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
human ... NCOA2(10499)
特異性
Clone 3Ti-3C11 mouse monoclonal antibody that detects NCoA-2. It targets an epitope within 246 amino acids from the internal part of the protein.
免疫原
His-tagged, recombinant, human NCoA-2 protein fragment (aa 624-1287).
應用
Quality Control Testing
Evaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Tested Applications
ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).
Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Tested Applications
ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).
Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Nuclear receptor coactivator 2 (UniProt: Q15596; also known as NCoA-2, Transcriptional intermediary factor 2, hTIF2, Class E basic helix-loop-helix protein 75 (bHLHe75)) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID: 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) functions as a transcriptional coactivator in various cellular processes. It belongs to the p160/steroid receptor coactivator (SRC) family of proteins. NCoA-2 plays a crucial role in the regulation of gene expression by interacting with nuclear receptors and other transcription factors. It possesses multiple functional domains, including a nuclear receptor interaction domain (NRID), a histone acetyltransferase (HAT) domain, and several transcription activation domains (ADs). NCoA-2 acts as a bridge between transcription factors and the transcriptional machinery, facilitating the recruitment of coactivators and chromatin remodeling complexes to target gene promoters. This coactivator function enables NCoA-2 to modulate diverse biological processes such as development, metabolism, cell proliferation, and differentiation. Dysregulation of NCoA-2 has been implicated in various diseases, including cancer, where it can promote tumor growth and progression.
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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