MABC1609

Anti-AIF Antibody, clone 3C11

• 别名: Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8
• UNSPSC代码: 12352203
• eCl@ss: 32160702

属性

• 生物来源: mouse
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 种属反应性: human
• 技术: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG1κ
• UniProt登记号: O95831
• 运输: ambient
• 基因信息: human ... AIFM1(9131)

说明

一般描述

Apoptosis-inducing factor 1, mitochondrial (UniProt: O95831; also known as Programmed cell death protein 8) is encoded by the AIFM1 (also known as AIF, PDCD8) gene (Gene ID: 9131) in human. AIF is a nuclear encoded flavoprotein that is generally confined to the mitochondrial intermembrane space and functions both as NADH oxidoreductase and as regulator of apoptosis. Six isoformsi of AIF have been described that are produced by alternative splicing. Under normal conditions, a 54-residue N-terminal (transit peptide) segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase to form the inner-membrane-anchored mature form (AIFmit). Upon induction of apoptosis, it is further proteolytically processed at to generate the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. AIF also functions as an anti-apoptotic factor in normal mitochondria via its NADH oxidoreductase activity. Once released from mitochondria it translocates to the nucleus to induce caspase-independent fragmentation of chromosomal DNA. Mutations in AIF gene are linked to combined oxidative phosphorylation deficiency 6 that is characterized by psychomotor delay, hypotonia, areflexia, and muscle weakness and wasting. Defects in this gene are also known to cause Cowchock syndrome that leads to early childhood onset of a slowly progressive axonal sensorimotor neuropathy.

特异性

Clone 3C11 specificallly detects AIF in human cells.

免疫原

His-tagged full length recombinant human AIF.

应用

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected AIF in human skin, human testis, and human kidney tissue.

Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in A431 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected AIF in 10 µg of A431 cell lysates.

外形

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.