MAB3785

Anti-APC Antibody, NT, clone Ali 12.28

clone Ali 12.28, Chemicon^®, from mouse

• 别名: Adenomatous Polyposis Coli Protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: Ali 12.28, monoclonal
• 物種活性: human
• 應無反應活性: mouse
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NM_000038.3
• UniProt登錄號: P25054
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... APC(324) ; mouse ... Apc(11789)

说明

一般說明

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

特異性

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

免疫原

Epitope: N-terminus

N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

聯結

Replaces: AB1268

外觀

Format: Purified

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

儲存和穩定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

分析報告

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable