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IPFL00010

Millipore

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

别名:

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

分類程式碼代碼:
41105339
eCl@ss:
32031602
NACRES:
NB.22

产品名称

Immobilon®-FL PVDF膜, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

材料

PVDF membrane
plain filter
white filter

品質等級

400

特點

hydrophobic

製造商／商標名

Immobilon®

技術

dot blot: suitable
western blot: suitable

過濾器長度 × 寬度

27 cm × 3.75 m

孔徑

0.45 μm pore size

容量

155 μg/cm2 adsorption capacity (insulin)
 205 μg/cm2 adsorption capacity (BSA)
 300 μg/cm2 adsorption capacity (goat IgG)

相容性

for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

檢測方法

chemiluminescent
colorimetric
fluorometric

運輸包裝

ambient

相关类别

一般說明

Immobilon®-FL膜是一种聚偏氟乙烯(PVDF)微孔膜，标称孔径为0.45 μm。它被设计用来结合从各种凝胶基质转移的蛋白质。Immobilon®-FL转移膜是疏水的，采用均匀的孔隙结构，具有高生物分子结合能力。该膜为分子量大于20,000的蛋白质提供了最佳印迹。Immobilon®-FL膜在很宽的激发/发射波长范围内显示非常低的自荧光。这种特性使其成为包括荧光免疫检测在内的印迹应用的理想材料。Immobilon®-FL膜也可用于标准化学发光或显色检测。

應用

Immobilon-FL PVDF膜已用于蛋白质印迹分析。

特點和優勢

Immobilon-FL膜：
  • 首款为荧光应用优化的转移膜；极低的背景提高了所有荧光检测协议的灵敏度
  • 在广泛的激发/发射波长范围内兼容所有常用的荧光探针
  • 最适合多路复用和化学荧光检测
  • 兼容标准阻断剂和缓冲液

法律資訊

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

WB检测样本前处理：细胞裂解和蛋白提取

Western blotting前用于培养组织和细胞的细胞裂解和有效蛋白提取的样品制备实验方案。

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