推荐产品
形狀
slurry (Liquid)
包含
≤0.1% sodium azide as preservative
製造商／商標名
Calbiochem®
儲存條件
do not freeze
技術
protein purification: suitable
適合性
suitable for microbiology
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭，以减少非特异性结合，不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。
應用
免疫沉淀法（参见评论）
警告
毒性：标准处理（A）
外觀
PBS 中 33％ 的浆液。
其他說明
提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭，不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应，建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Lixuan Zhan et al.
Journal of neuroinflammation, 18(1), 97-97 (2021-04-22)
Our previous study indicated that hypoxic preconditioning reduced receptor interacting protein (RIP) 3-mediated necroptotic neuronal death in hippocampal CA1 of adult rats after transient global cerebral ischemia (tGCI). Although mixed lineage kinase domain-like (MLKL) has emerged as a crucial molecule
Ji Hyun Shin et al.
Autophagy, 15(9), 1495-1505 (2019-03-02)
Several studies have shown that dysfunction of macroautophagy/autophagy is associated with many human diseases, including neurodegenerative disease and cancer. To explore the molecular mechanisms of autophagy, we performed a cell-based functional screening with SH-SY5Y cells stably expressing GFP-LC3, using an
Yi Guan et al.
Journal of the American Society of Nephrology : JASN, 28(8), 2337-2352 (2017-03-02)
The rapid growth of an aging population creates challenges regarding age-related diseases, including AKI, for which both the prevalence and death rate increase with age. The molecular mechanism by which the aged kidney becomes more susceptible to acute injury has
Abdulaziz Asiri et al.
Pathology international, 69(7), 381-391 (2019-07-11)
Cten is an oncogene promoting EMT in many signaling pathways, namely through Snail. We investigated whether Cten function could be mediated through Src. Cten levels were modulated by forced expression in HCT116 and gene knockdown in SW620 CRC (colorectal cancer)
Kylene Kehn et al.
Retrovirology, 1, 6-6 (2004-06-01)
The human T-cell leukemia virus type 1 (HTLV-1) Tax protein indirectly influences transcriptional activation, signal transduction, cell cycle control, and apoptosis. The function of Tax primarily relies on protein-protein interactions. We have previously shown that Tax upregulates the cell cycle
相关内容
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门