IM42

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

liquid, clone 114-6G6, Calbiochem^®

• 别名: Anti-MMP-14
• UNSPSC代码: 12352203

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: 114-6G6, monoclonal
• 表单: liquid
• 包含: ≤0.1% sodium azide as preservative
• 种属反应性: human
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze; avoid repeated freeze/thaw cycles
• 同位素/亚型: IgG1
• 运输: wet ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... MMP14(4323)

说明

一般描述

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.

Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

免疫原

a synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP

应用

Frozen Sections (not recommended)
Paraffin Sections (10 g/ml, Pronase<TMSYMBOL></TMSYMBOL> or heat pre-treatment required)
Immunoblotting (10 g/ml)
Immunocytochemistry (see application reference)
Immunoprecipitation (not recommended)

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

外形

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Negative Control
trpE (Ab-1) or untreated MD-MB-231 cells

Positive Control
HT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue

其他说明

Does not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.

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Okada, A., et al. 1997. J. Cell. Biol.147, 67.
Okumura, Y., et al. 1997. FEBS Lett.402, 181.
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Strongin, A.Y., et al. 1995. J. Biol. Chem.270, 5331.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Sato, H., et al. 1994. Nature370, 61.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Fridman, R., et al. 1992. J. Biol. Chem.267, 15389.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
Goldberg, G.I., et al. 1989. PNAS86, 8207.

法律信息

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1