生物源
rabbit
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
do not freeze
同型
IgG
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CTSD(1509)
一般說明
兔抗组织蛋白酶D(Ab-2)多克隆抗体识别乳腺癌组织中组织蛋白酶D的~52 kDa潜态(latent)形式和 ~34 kDa亚基。它经过验证可用于冷冻和石蜡切片的WB和IHC分析。
可识别乳腺癌组织提取物中组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的亚基，但不可识别〜14 kDa亚基。
纯化的兔多克隆抗体。可识别组织蛋白酶D〜52 kDa的潜在形式和〜34 kDa的（但不包括〜14 kDa）活性亚基。
免疫原
人
纯化的人肝组织蛋白酶D
應用
冷冻切片（5 µg/ml）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
免疫印迹（2.5 µg/ml）
石蜡切片（5 µg/ml，需要胰蛋白酶预处理）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外觀
溶于50 mM磷酸钠缓冲液（pH 7.5），含0.2％明胶。
分析報告
阳性对照
HT1080浓缩条件培养基或乳腺癌组织
HT1080浓缩条件培养基或乳腺癌组织
其他說明
Rochefort, H. 1992.Eur. J. Cancer28A, 1780.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
Rochefort, H. 1990.In Seminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1(2), 153.
Westly, B. and Rochefort, H. 1980.Cell20, 352.
Barret, A. J. 1977.In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
为了获得最佳的免疫印迹结果，应在上样前将HT1080细胞的条件培养基浓缩10-20X。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10-13 - German Storage Class 10 to 13
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