HTS156M

ChemiSCREEN Membrane Preparation Recombinant Human MC ₁ Melanocortin Receptor

• 分類程式碼代碼: 41106514
• eCl@ss: 32161000
• NACRES: NA.45

属性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in Chem-1 cells
• 形狀: liquid
• 製造商／商標名: ChemiScreen; Chemicon^®
• 技術: ligand binding assay: suitable (GTPγS); radioligand binding assay (RLBA): suitable
• NCBI登錄號: NM_002386.2
• UniProt登錄號: Q01726

说明

一般說明

The melanocortin-1 receptor (MC₁) is a member of the G-protein-coupled receptor family of proteins that is responsible for regulating skin and hair color. The MC₁ receptor is regulated by the physiological agonists alpha-melanocyte-stimulating hormone (alphaMSH) and adrenocorticotropic hormone (ACTH), and antagonist agouti signaling protein (ASP) (Rouzud et al., 2005). In 1995 a landmark study demonstrated that over 80% of humans with red hair or fair skin have a dysfunctional variant of the MC₁ gene (Valverde et al., 1995). MC₁ is, therefore, a major determinant of sun sensitivity and genetic risk factor for melanoma and non-melanoma skin cancer. Millipore′s MC₁ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of MC₁. The membrane preparations exhibit a Kd of 1.3nM for [¹²⁵I]-[Nle⁴, D-Phe⁷]-α-MSH (NDP-αMSH). With 0.3 nM [¹²⁵I]-NDP-αMSH, 5μg/well MC₁ Membrane Prep typically yields greater than 50-fold signal-to-background ratio.

應用

Human MC1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

Radioligand binding assay and GTPγS binding.

生化／生理作用

• GPCR Class: A
• Protein Target: MC1
• Target Sub-Family: Melanocortin

外觀

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to 1 mg/mL in packaging buffer, and dispensed at 1 mL/vial. Vials were rapidly frozen, and stored at -80°C.

儲存和穩定性

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2