一般描述
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001). CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005). CXCR6 functions as a cofactor with CD4 for HIV entry and Env-mediated cell fusion (Liao et al., 1997). Binding of CXCL16 to CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Millipore′s cloned human CXCR6 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CXCR6 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR6.
The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001). CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005). CXCR6 functions as a cofactor with CD4 for HIV entry and Env-mediated cell fusion (Liao et al., 1997). Binding of CXCL16 to CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Millipore′s cloned human CXCR6 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CXCR6 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR6.
细胞系描述
- GPCR Cell Lines
- Host cells: Chem-5
应用
Human CXCR6 GPCR frozen cells for Calcium Flux FLIPR Assays.
生化/生理作用
- GPCR Class: A
- Protein Target: CXCR6
- Target Sub-Family: Chemokine
组分
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
免责声明
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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