ABE213

Anti-monomethyl-Histone H3 (Lys79) Antibody

from rabbit, purified by affinity chromatography

• 别名: Histone H3.1t, H3/g
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, rat, human
• 技術: dot blot: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_003484.1
• UniProt登錄號: Q16695
• 運輸包裝: wet ice
• 目標翻譯後修改: monomethylation (Lys79)
• 基因資訊: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

说明

一般說明

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

特異性

Predicted to react with multiple vertebrate species due to sequence conservation.

This antibody recognizes Histone H3 methylated at Lys79. Using peptide dot blot analysis slight cross-reactivity with dimethyl-Histone H3 (Lys79) and unmodified Histone H3 (Lys79) may be observed. Using peptide inhibition analysis assay no cross reactivity to these peptides is observed.

免疫原

Epitope: Methylated at Lys79.

KLH-conjugated linear peptide corresponding to human Histone H3 monmethylated at Lys79.

應用

Dot Blot Analysis: A representative lot detected monomethyl-Histone H3 in Dot Blot.

Peptide Inhibition Analysis: A representative lot blocked monomethyl-Histone H3 in HeLa acid extract.

Immunoprecipitation Analysis: A representative lot detected monomethyl-Histone H3 in HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-monomethyl-Histone H3 (Lys79) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, PIA, IP to detect monomethyl-Histone H3 (Lys79) also known as H3K79me1, Histone H3 (mono methyl K79).

品質

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:500 dilution of this antibody detected monomethyl-Histone H3 in HeLa acid extract.

標靶描述

~17 kDa observed

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa acid extract.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable