ABC961

Anti-OFD1 Antibody

from rabbit, purified by affinity chromatography

• 别名: Oral-facial-digital syndrome 1 protein homolog, OFD1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, human
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_803178
• UniProt登錄號: Q80Z25
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Ofd1(302661)

说明

一般說明

The cilium is a cell surface projection found in many vertebrate cells and it is required to transduce signals important for development and tissue homeostasis. OFD1 is important in cilium development, and thus it is important in cellular development and signaling. OFD1 is a component of the centrioles and appears to control the length of the centrioles in mother-daughter cells. OFD1 also plays an important role in the WNT signaling pathway and in the formation of the left-right axis in embryos. OFD1 is expressed in the cytoplasm during development in most tissues but can be found in the nucleus as well, and it is known to interact with proteins important in chromatin remodeling as well. At least one X-linked genetic mutation is associated with defects in the OFD1 gene. It is a form of oroffaciodigital syndrome characterized by abnormalities in the oral cavity, face, digits and other disfigurements that is lethal in males.

免疫原

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of mouse OFD1.

應用

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-OFD1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of OFD1.

Western Blotting Analysis: A representative lot detected OFD1 in untreated and autophagy-induced/serum-starved mouse MEFs (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).

品質

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.

標靶描述

~120 kDa observed. Uncharacterized bands may be observed in some lysates at 45 kDa, 33 kDa & 28 kDa

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable