跳转至内容
Merck
所有图片(2)

主要文件

AB2999

Sigma-Aldrich

Anti-BNIP3 Antibody

from rabbit, purified by affinity chromatography

别名:

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

登录查看公司和协议定价


About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
無性繁殖:
polyclonal
application:
ICC
WB
物種活性:
human
技術:
immunocytochemistry: suitable
western blot: suitable
citations:
2

生物源

rabbit

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

物種活性(以同源性預測)

mouse (based on 100% sequence homology)

技術

immunocytochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... BNIP3(664)

一般說明

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family, a singular group of death-inducing, mitochondrial proteins. BNIP3 is comprised of several domains of interest; a highly conserved domain (CD), a C-terminal transmembrane (TM) domain, and a BH3 domain. Strongly upregulated by hypoxia, BNIP3 induces a caspase-independent cell death characterized by mitochondrial damage. Overexpression has been observed in the brain after stroke and in the adult heart following myocardial infarction. Recent studies have shown BNIP3 to be involved in cell growth inhibition through suppression of the mTOR pathway and that, when localized to the nucleus, it has the paradoxical capability of repressing apoptosis in tumors by binding the AIF expression gene.

特異性

Other homologies: Rat, equine, and ox (90% sequence homology).

免疫原

Epitope: Internal (C-terminal half).
KLH-conjugated linear peptide corresponding to an internal sequence within the C-terminal half of human BNIP3.

應用

Anti-BNIP3 Antibody detects level of BNIP3 & has been validated for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected hypoxia-induced BNIP3 upregulation in human glioblastoma-astrocytoma, epithelial-like U87 cells (Courtesy of Spencer Gibson, Manitoba Institute of Cell Biology).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

品質

Evaluated by Western Blotting in PC3 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected BNIP3 in 10 µg of PC3 cell lysate.

標靶描述

~60 kDa (dimer) observed. BNIP3 may be observed as a 30 kDa monomer and/or a 60 kDa dimer.

外觀

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
PC3 cell lysate.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Y Yuan et al.
Cell death & disease, 4, e690-e690 (2013-06-29)
The histone methyltransferase G9a is overexpressed in a variety of cancer types, including pancreatic adenocarcinoma, and promotes tumor invasiveness and metastasis. We recently reported the discovery of BRD4770, a small-molecule inhibitor of G9a that induces senescence in PANC-1 cells. We
Lisa A Brennan et al.
Experimental eye research, 174, 173-184 (2018-06-08)
The formation and life-long growth of the ocular lens depends on the continuous differentiation of lens epithelial cells into lens fiber cells. To achieve their mature structure and transparent function, newly formed lens fiber cells undergo a series of cellular

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门