616430

TGF-α, Human, Recombinant, E. coli

• 别名: TGF-α, Human, Recombinant, E. coli, Transforming Growth Factor-α
• CAS号: 105186-99-0
• MDL编号: MFCD00082077
• UNSPSC代码: 12352202

属性

• 方案: ≥97% (SDS-PAGE)
• 质量水平: 100
• 表单: lyophilized
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze
• 杂质: ≤100 pg/μg Endotoxin (pg/μg TGF-α)
• 运输: dry ice
• 储存温度: −70°C

说明

一般描述

Recombinant, human transforming growth factor-α expressed in E. coli. Plays an important role in cell-cell adhesion and cell growth and differentiation. Reported to play a role in the development of hormonal insensitivity in estrogen-receptor-positive breast cancers.

生化/生理作用

ED₅₀= 0.1-0.4 ng/ml as measured by the ability to stimulate ³H-thymidine incorporation in the mouse fibroblast cell line Balb/3T3

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from 30% acetonitrile, 0.1% TFA.

重悬

Following reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile 10 mM acetic acid containing ≥0.1% HSA or BSA.

其他说明

Nicholson, R.I., et al. 1994. Cancer Res. 54, 1684.
Anklesaria, P., et al. 1990. Proc. Natl. Acad. Sci. USA87, 3289.
Massague, J. 1990. J. Biol. Chem.265, 21393.
Watanabe, S., et al. 1987. Proc. Natl. Acad. Sci. USA84, 1258.
Schreiber, A.B., et al. 1986. Science232, 1250.
Tam, J.P. 1985. Science229, 673.
Derynck, R., et al. 1984. Cell38, 327.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany