5.38299

M2I-1

• 别名: M2I-1, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad 2 Inhibitor- 1, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydro-4,6(1H,5H)-pyrimidinedione, 5-(4-(Diisobutylamino)-3-nitrobenzylidene)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione, Mad2Inhibitor-1
• 经验公式（希尔记法）: C₁₉H₂₄N₄O₄S
• 分子量: 404.48
• 分類程式碼代碼: 12352200

属性

• 化驗: ≥98% (HPLC)
• 品質等級: 100
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 顏色: orange
• 溶解度: DMSO: 50 mg/mL
• 儲存溫度: 2-8°C

说明

一般說明

A cell-permeable nitrobenzylidene-thiobarbiturate compound that directly targets the hydrophobic core of Mad2 and selectively disrupts Mad2-Cdc20 complex formation. Shown to dose-dependently inhibit the interaction of Mad2 to Mad2-binding-peptide (Mbp1; IC₅₀ ~ 10.0 µM & K_d = 200 nM). Suggested to covalently modify Mad2-Cys¹⁴⁹ via a reversible Michael adduct formation. Significantly reduces the mitotic duration in paclitaxel (0.5 µM; Cat. No. 580555)-treated HeLa cells (25 µM of M2I-1) and impairs mitotic spindle assembly checkpoint (SAC) function.

A cell-permeable nitrobenzylidene-thiobarbiturate compound that directly targets the hydrophobic core of Mad2 and selectively disrupts Mad2-Cdc20 complex formation. Shown to dose-dependently inhibit the interaction of Mad2 to Mad2-binding-peptide (Mbp1; IC₅₀ ~ 10.0 µM & K_d = 200 nM). Suggested to covalently modify Mad2-Cys¹⁴⁹ via a reversible Michael adduct formation. Significantly reduces the mitotic duration in paclitaxel (0.5 µM; Cat. No. 580555)-treated HeLa cells (25 µM of M2I-1) and impairs mitotic spindle assembly checkpoint (SAC) function.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

生化／生理作用

Cell permeable: yes

Primary Target
Mad2

Reversible: yes

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Use only fresh DMSO for reconstitution.

其他說明

Kastl, J., et al. 2015. ACS Chem. Biol.10, 1661.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable