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物種活性
human, rat, mouse
品質等級
製造商/商標名
Milliplex®
技術
multiplexing: suitable
檢測方法
fluorometric (Luminex® xMAP®)
儲存溫度
2-8°C
一般說明
The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-622MAG) has been developed for the simultaneous detection of phosphorylated JNK (Thr183/Tyr185) and total JNK in a single well using the Luminex system.
特異性
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
應用
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: Erk (Total), Erk (Thr185/Tyr187).This is an overnight (4°C) incubation assay.This kit must be run using the provided Assay Buffer.This assay requires 25 μL diluted filtered cell lysate per well.The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1,000 μg/mL).Analytes available:JNK (Total);JNK (Thr183/Tyr185)
包裝
96-well plate
法律資訊
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
相关内容
MILLIPLEX® cell signaling assays facilitate detection of phosphoproteins and total proteins in cellular pathways.
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