480450

NFF-2

A quenched fluorescence substrate for MMPs.

• 别名: NFF-2, MCA-RPKPYANvaWMK(DNP)-NH₂
• 经验公式（希尔记法）: C₇₉H₁₀₅N₁₉O₁₉S
• 分子量: 1656.86
• 分類程式碼代碼: 12352200
• NACRES: NA.77

属性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated; protect from light
• 顏色: yellow
• 溶解度: DMSO: 5 mg/mL
• 螢光: λ_ex ~325 nm; λ_em ~393 nm
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

A quenched fluorescence substrate for MMPs. MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 5.9 x 10⁴ M^-1sec^-1) at a rate 60 times faster than the hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 5 x 10⁴ M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 5.5 x 10⁴ M^-1s^-1).

A quenched fluorescent substrate for MMPs (excitation maximum: ~325 nm; emission maximum: ~393 nm). MMP-3 (stromelysin-1) hydrolyzes this substrate (k_cat/K_m = 59,400 M^-1s^-1) at a rate 60X faster than hydrolysis by MMP-1 (interstitial collagenase). NFF-2 shows little discrimination between MMP-3, MMP-2 (gelatinase-A; k_cat/K_m = 54,000 M^-1s^-1), and MMP-9 (gelatinase-B; k_cat/K_m = 55,300 M^-1s^-1).

生化／生理作用

Cell permeable: no

Primary Target
Matrix Metalloproteinases (MMPs)

Product does not compete with ATP.

Reversible: no

kcat/Km = 5.9 x 104 M-1sec-1 as substrates for MMP-3 (stromelysin-1)

警告

Toxicity: Standard Handling (A)

序列

MCA-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met-Lys(DNP)-NH₂ (DNP = 2,4-dinitrophenyl; Nva = L-norvaline)

外觀

Supplied as a trifluoroacetate salt.

重構

Following initial thaw, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Nagase, H., et al. 1994. J. Biol. Chem. 269, 20952.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable