推荐产品
生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
R1JHL, monoclonal
形狀
lyophilized
不包含
preservative
物種活性
rat
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.
免疫原
Rat
a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
應用
Immunoblotting (1:300, colorimetric)
Immunoprecipitation (3 µg/200 µg lysate)
Immunoprecipitation (3 µg/200 µg lysate)
警告
Toxicity: Standard Handling (A)
外觀
Lyophilized from cell culture supernatant.
重構
Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
分析報告
Positive Control
Rat cortex tissue extracts
Rat cortex tissue extracts
其他說明
Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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