407252

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

• 别名: mIGF-I
• UNSPSC代码: 12352202

属性

• 方案: ≥98% (SDS-PAGE and HPLC)
• 质量水平: 100
• 表单: lyophilized
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze
• 杂质: ≤1 ng/μg Endotoxin (ng/μg IGF-I)
• 运输: wet ice
• 储存温度: −20°C

说明

一般描述

Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.

生化/生理作用

ED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.

警告

Toxicity: Standard Handling (A)

外形

Sterile-filtered prior to lyophilization. Contains no additives.

重悬

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.

Reconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.

其他说明

Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res.14, 7873.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable