生物源
rabbit
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human, canine, mouse, rat, porcine, bovine
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)
一般說明
Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.
免疫原
a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
應用
ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
警告
Toxicity: Standard Handling (A)
外觀
In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
重構
Following initial thaw, aliquot and freeze (-20°C).
其他說明
Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Variables associated with assay conditions will dictate the proper working dilution.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies
NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.
• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent
1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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