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Merck
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主要文件

375230

Sigma-Aldrich

HGF, Mouse, Recombinant

HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

别名:

Hepatopoietin A

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About This Item

UNSPSC代码:
12352203

质量水平

方案

≥95% (as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions., SDS-PAGE)

表单

solid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze

杂质

<1.0 EU/μg protein Endotoxin

运输

ambient

储存温度

−20°C

一般描述

Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

生化/生理作用

Cell Proliferation:

ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.



Functional ELISA

KD<0.2 nM as measured by binding to C-Met (HGF Receptor).

警告

Toxicity: Standard Handling (A)

重悬

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

其他说明

Corso, S., et al. 2005. Trends Mol. Med.11, 284.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3


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