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Merck
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17-283

Sigma-Aldrich

Rac1活化测定试剂盒

Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.

别名:

Activation Assay Kit, Rac1 Activity Kit, Rac1 Assay Kit

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.32

品質等級

物種活性

human, rat, mouse

製造商/商標名

Upstate®

技術

activity assay: suitable
affinity binding assay: suitable

UniProt登錄號

運輸包裝

dry ice

基因資訊

human ... RAC1(5879)

一般說明

非放射性Rac1活化测定试剂盒可用于沉淀细胞裂解物中的Ras相关C3肉毒杆菌毒素底物1 (Rac1)-GTP,并通过Rac1特异性单克隆抗体检测。Rac1属于小鸟苷三磷酸酶(GTPases)的Ras超级家族。它以两种主要形式表达,即非活性GDP结合形式和活性GTP结合形式。Rac1通过与激活多种信号级联的效应物相互作用,参与影响多种生理条件。RAC1基因位于人染色体7p22.1。

應用

Rac1激活测定试剂盒已用于通过免疫印迹法检测小鼠和人脑匀浆中的活性Ras相关C3肉毒杆菌毒素底物1 (Rac1)。

成分

抗Rac1,克隆23A8(目录号05-389)

Mg2+裂解/洗涤缓冲液,5X(目录号20-168)

100X GTPγS,10mM(目录号20-176)

100X GDP,100mM(目录号20-177)

Rac/Cdc42测定试剂(PAK1 PBD,琼脂糖)(目录号14-325)

品質

通过从已加载GTPγS的3T3细胞裂解物中沉淀Rac-GTP进行常规评估。 使用抗Rac通过免疫印迹分析检测沉淀的Rac-GTP。

聯結

替代:SGT425

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

CorrosionEnvironment

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

儲存類別代碼

10 - Combustible liquids


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The role of mechanical stretching in the activation and localization of adhesion proteins and related intracellular molecules.
Boccafoschi, F, et al.
Journal of Cellular Biochemistry, 112, 1403-1409 (2011)
rs12977 of RAC1 is associated with the progress of gastric cancer in Iranian population
Javani A, et al.
Gene Reports, 23, 101069-101069 (2021)
The survival kinase Mirk/dyrk1B is activated through Rac1-MKK3 signaling.
Kideok Jin, Seunghwan Lim, Stephen E Mercer, Eileen Friedman
The Journal of Biological Chemistry null
Incorporation of CXCR4 into membrane lipid rafts primes homing-related responses of hematopoietic stem/progenitor cells to an SDF-1 gradient.
Marcin Wysoczynski, Ryan Reca, Janina Ratajczak, Magda Kucia, Neeta Shirvaikar et al.
Blood null
Michelle E Marchese et al.
Biochemistry, 51(41), 8235-8246 (2012-09-14)
Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for

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