推荐产品
product name
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
材料
PEEK column
品質等級
agency
suitable for USP L8
產品線
Chromolith®
特點
endcapped: no
包裝
1 ea of
標籤範圍
5% Carbon loading
參數
200 bar pressure
45 °C max. temp.
技術
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
柱長度 × 內徑
10 cm × 4.6 mm
基質
monolithic silica
基質活性組
NH2 bonding phase
孔徑
13 nm mesoporosity
2 μm macroporosity
工作pH值範圍
2-7.5
分離技術
hydrophilic interaction (HILIC)
normal phase
reversed phase
儲存溫度
no temp limit
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相关类别
一般說明
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
分析報告
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
法律資訊
商品
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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