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Merck
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主要文件

14-752-M

Sigma-Aldrich

GRK7 Protein, active, 10 µg

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.32

生物源

human

品質等級

100
300

分子量

Mw 66 kDa

製造商/商標名

Upstate®

技術

activity assay: suitable (kinase)

NCBI登錄號

UniProt登錄號

一般說明

N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.
Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

應用

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
Research Category
Metabolism
Research Sub Category
Cardiovascular Disease|Metabolic Disorders

生化/生理作用

  • Protein Target: GRK7
  • Target Sub-Family:AGC

品質

Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.

單位定義

Specific Activity: where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.

外觀

10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Format: Purified using Ni2+/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.

儲存和穩定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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