14-751-M

Abl (Q252H) Protein, active, 10 µg

Active, N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. For use in Kinase Assays.

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 100; 300
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 比活性: 1089 U/mg
• 分子量: M_w 121 kDa
• 製造商／商標名: Upstate^®
• 濃度: 51% (Coomassie staining, SDS-PAGE)
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_005157.3; NM_007313.2
• UniProt登錄號: P00519

说明

一般說明

N-terminal 6His-tagged recombinant human Abl, residues 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).

Product Source: Expressed by baculovirus in Sf21 insect cells.

應用

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化／生理作用

Protein Target: Abl

Target Sub-Family: TK

品質

Routinely evaluated by phosphorylation of 50 μM Abltide. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active Abl (Q252H).

單位定義

Specific Activity: one unit of Abl (Q252H) activity is defined as 1 nmol phosphate incorporated into 50 μM Abltide (EAIYAAPFAKKK) per minute at 30°C with a final ATP concentration of 100 μM.

外觀

Ni²⁺/NTA-agarose

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 150 mM NaCl, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.

儲存和穩定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable