14-749-M

TAO1 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.

• UNSPSC代码: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物来源: human
• 质量水平: 100
• 重组: expressed in baculovirus infected Sf21 cells
• 方案: 93% (Coomassie blue staining, SDS-PAGE)
• 比活: 1179 U/mg
• 分子量: M_w 41 kDa
• 制造商/商品名称: Chemicon^®
• 技术: activity assay: suitable (kinase)
• NCBI登记号: NM_020791.1
• UniProt登记号: Q7L7X3
• 基因信息: human ... TAOK1(57551)

说明

一般描述

N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327.

Product Source: Expressed by baculovirus in Sf21 cells.

应用

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化/生理作用

Protein Target: TAO1

Target Sub-Family: STE

质量

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1.

单位定义

Specific Activity: where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

外形

Ni²⁺/NTA-agarose

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

储存及稳定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.