14-744-M

Haspin Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 100; 300
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 比活性: 2237 U/mg
• 分子量: M_w 41.1 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_031965.2
• UniProt登錄號: Q8TF76

说明

一般說明

N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.

Product Source: Recombinant enzyme expressed in Sf21 cells

應用

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化／生理作用

Protein Target: haspin

Target Sub-Family: Misc

品質

Routinely evaluated by phosphorylation of 500 μM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.

單位定義

Specific Activity: where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.

外觀

Purified using Ni²⁺/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

儲存和穩定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable