14-743-M

GCK Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

• 别名: germinal center kinase
• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 比活性: 2337 U/mg
• 分子量: M_w 80.6 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_004579.2
• UniProt登錄號: Q12851

说明

一般說明

N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.

Product Source: Recombinant protein expressed in Sf21 cells

應用

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

生化／生理作用

Protein Target: GCK

Target Sub-Family: STE

品質

Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.

單位定義

Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.

外觀

Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.

儲存和穩定性

Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.