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Merck
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主要文件

14-1054

Sigma-Aldrich

Human recombinant histone H3.3-H4 tetramers

Human recombinant histone H3.3-H4 tetramers expressed in E Coli as fusion proteins with either an N-terminal FLAG or 6xHis epitopes & purified by Fast Protein Liquid Chromatography (FPLC).

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About This Item

分類程式碼代碼:
12352200
eCl@ss:
32160405
NACRES:
NA.32

生物源

Escherichia coli

重組細胞

expressed in E. coli

化驗

>98% (SDS-PAGE)

製造商/商標名

Upstate®

濃度

1 μg/μL

技術

activity assay: suitable

運輸包裝

dry ice

一般說明

Human recombinant histone H3.3-H4 tetramers expressed in E Coli. as fusion proteins with either an N-terminal FLAG® or 6xHis epitopes, and purified by Fast Protein Liquid Chromatography (FPLC).
Product Source: Human
The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The histone variant H3.3 replaces the conventional H3.1 histone in a wide range of nucleosomes in active genes, and are incorporated into chromatin independently of DNA synthesis. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP) which requires high quality chromatin and essential tools such as histone proteins and appropriate enzymes to assemble chromatin in vitro.

應用

Research Category
Epigenetics & Nuclear Function
Suitable for in vitro chromatin assembly and enzyme activity assays

外觀

In buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 2 M NaCl, 0.5 mM PMSF and 1 mM DTT

儲存和穩定性

Stable at -80ºC for 1 year from date of receipt.

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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