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一般說明
髓过氧化物酶（EC 1.11.2.2；UniProt P05164）由人的MPO基因（基因ID 4353）编码。髓过氧化物酶/MPO是在髓样分化过程中合成的血红素蛋白，构成嗜中性粒细胞嗜天青颗粒的主要成分。髓过氧化物酶以具有信号肽序列的745 a.a.单链前体（a.a.1-48）生成，随后被切割成轻链（a.a.165-278）和重链（a.a.279-745）。成熟的髓过氧化物酶是由2条轻链和2条重链组成的四聚体。MPO催化过氧化氢依赖性氧化和次氯酸的形成，而次氯酸对嗜中性粒细胞的杀微生物活性至关重要。
特異性
免疫原序列存在于髓过氧化物酶的前体和成熟形式中（无a.a.1-48，a.a.1-154，或a.a.1-164），以及裂解的重链中（a.a.279-745），但不存在轻链中（a.a.165-278）。
免疫原
对应于人髓过氧化物酶重链区域内序列的KLH偶联线性肽。
應用
抗髓过氧化物酶抗体（目录号07-496）是一种高度特异性的兔多克隆抗体，靶向髓过氧化物酶，并通过流式细胞术和蛋白质印迹法进行了测试。
流式细胞分析：0.1 µg代表性批次在4%多聚甲醛固定的0.2%Triton X-100透化的HL-60细胞中检测到髓过氧化物酶免疫反应性。
品質
通过蛋白质印迹对Jurkat细胞裂解液进行了评估。
蛋白质印迹分析：1 µg/mL的该抗体在10 µg Jurkat细胞裂解液中检测到髓过氧化物酶重链。
蛋白质印迹分析：1 µg/mL的该抗体在10 µg Jurkat细胞裂解液中检测到髓过氧化物酶重链。
標靶描述
观察到约92/60 kDa（糖基化前体/重链）。83.87/73.85/87.25 kDa（亚型H17/H14/H7前体），78.94/82.32 kDa（亚型H17/H7，去除a.a.1-48前肽），66.45/66.45/69.83 kDa（亚型H17/H14/H7，去除a.a.1-154），65.48/65.48/68.86 kDa（亚型H17/H7亚型H17/H14/H7，去除a.a.1-164），52.51 kDa（重链；a.a.279-745）。在一些裂解液中可能会观察到未表征的条带。
聯結
替代：07-496
其他說明
浓度：请参考特定批次的数据表。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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