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06-852

Sigma-Aldrich

抗-Spinophilin/Neurabin-II抗体

Upstate®, from rabbit

别名:

Protein phosphatase 1 regulatory subunit 9B 3 neurabin II, protein phosphatase 1, regulatory (inhibitor) subunit 9B, protein phosphatase 1, regulatory subunit 9B, protein phosphatase 1, regulatory subunit 9B, spinophilin

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

rat

製造商/商標名

Upstate®

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... PPP1R9B(84687)

一般說明

Spinophilin/neurabin II是一种肌动蛋白相关的支架蛋白,富含神经元的树突棘。

特異性

其他物种未知。
特异于spinophilin,Mr 120 kDa。也可识别疑似为spinophilin(Mr 95kDa)裂解产物的条带。

免疫原

GST融合蛋白,对应人脑Spinophilin/Neurabin-II的286-390位残基。

應用

免疫沉淀:
4 μg的先前批次对来自500 μg大鼠脑微粒体制剂(目录号12-144)的Spinophilin/Neurabin-II进行免疫沉淀。
抗Spinophilin/Neurabin-II抗体是一种针对Spinophilin/Neurabin-II的抗体,可用于IC、IP & WB。
研究子类别
突触 & 突触生物学

神经元 & 胶质标记
研究类别
神经科学

品質

已通过蛋白免疫印迹对大鼠脑微粒体制剂进行了常规评估。

蛋白质印迹分析:
0.25-1 μg/mL该批次产品已在20 μg大鼠脑微粒体制剂(目录号12-144)中检测到Spinophilin/Neurabin-II。

標靶描述

120 kDa

外觀

形式:纯化
纯化的兔多克隆IgG,溶于含有70%储存缓冲液(0.014 M 磷酸盐缓冲液(pH 7.6)、0.175 M NaCl、0.07%叠氮化钠)和30%甘油的缓冲液中。储存于 -20°C。
蛋白G纯化

儲存和穩定性

自收到之日起在-20ºC可稳定保存1年。

处理建议:
收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。避免反复冻融,其可能会破坏IgG并影响产品性能。注意:冷藏室温度变化至低于-20°C时可能导致含甘油的溶液在储存过程中冻结。

分析報告

对照
脑组织

其他說明

浓度:请参考批次特异性浓缩物的分析证书。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Bridget M Nugent et al.
Nature neuroscience, 18(5), 690-697 (2015-03-31)
The developing mammalian brain is destined for a female phenotype unless exposed to gonadal hormones during a perinatal sensitive period. It has been assumed that the undifferentiated brain is masculinized by direct induction of transcription by ligand-activated nuclear steroid receptors.
A Satoh et al.
The Journal of biological chemistry, 273(6), 3470-3475 (1998-03-07)
In a preceding paper, we reported a novel actin filament (F-actin)-binding protein, named neurabin, which was specifically expressed in neural tissue and implicated in neurite formation. We purified from rat brain another F-actin-binding protein, which had a domain organization similar
Ryan T Terry-Lorenzo et al.
The Journal of biological chemistry, 277(48), 46535-46543 (2002-09-25)
Inhibitor-2 (I-2) bound protein phosphatase-1 (PP1) and several PP1-binding proteins from rat brain extracts, including the actin-binding proteins, neurabin I and neurabin II. Neurabins from rat brain lysates were sedimented by I-2 and its structural homologue, I-4. The central domain
Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.
Jun Xu
Brain Research Molecular Brain Research null
Anthony J Baucum et al.
Molecular & cellular proteomics : MCP, 9(6), 1243-1259 (2010-02-04)
Spinophilin regulates excitatory postsynaptic function and morphology during development by virtue of its interactions with filamentous actin, protein phosphatase 1, and a plethora of additional signaling proteins. To provide insight into the roles of spinophilin in mature brain, we characterized

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