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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
CS112-2A8, monoclonal
物種活性
mouse, human, rat
製造商／商標名
Upstate®
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... SFN(2810)
mouse ... Sfn(55948)
rat ... Sfn(313017)
特異性
Recognizes 14-3-3σ, does not cross-react with other 14-3-3 isoforms.
免疫原
GST fusion protein corresponding to full-length human 14-3-3 sigma
應用
Detect 14-3-3σ using this Anti-14-3-3σ Antibody, clone CS112-2A8 validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
品質
routinely evaluated by immunoblot on RIPA lysates of EGF-stimulated A431 cells
標靶描述
30 kDa
外觀
Protein G Purified
Format: Purified
Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
儲存和穩定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
A Santana et al.
Journal of dairy science, 106(9), 6060-6079 (2023-07-21)
The aim of this study was to evaluate the inclusion of alfalfa grazing during 8 h continuous or partitioned in 2 separated sessions of 4 h after each milking, on nutrient intake, digestibility, ruminal fermentation, feeding behavior, milk production, milk
Claudio A Pozo et al.
Journal of dairy science, 105(6), 4987-5003 (2022-04-23)
Our aim was to evaluate whether increasing soluble carbohydrates in the herbage by changing the time of the grazing session or including Acacia mearnsii tannin in the diet would affect intake, digestion, N partitioning, and productive performance of dairy cows
D E Oliveira et al.
Journal of dairy science, 95(3), 1437-1446 (2012-03-01)
Feeding conjugated linoleic acid (CLA) in a rumen-inert form to dairy ewes has been shown to increase milk production, alter milk composition, and increase the milk fat CLA content. However, few studies have tested ruminally unprotected CLA sources. The objective
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Journal of dairy science, 100(2), 1072-1085 (2016-12-13)
The objective of our study was to investigate the effects of sources of calcium salts of fatty acids (FA) on production, nutrient digestibility, energy balance, and carryover effects of early lactation grazing dairy cows. Treatment diets were offered from 3
J Zha et al.
The Journal of biological chemistry, 272(39), 24101-24104 (1997-09-26)
BAD interacts with anti-apoptotic molecules BCL-2 and BCL-XL and promotes apoptosis. BAD is phosphorylated on serine residues in response to a survival factor, interleukin-3. Phosphorylated BAD cannot bind to BCL-XL or BCL-2 at membrane sites and is found in the
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