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Merck
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05-577

Sigma-Aldrich

抗-PP2A抗体,C 亚基,去甲基化,克隆 4b7

ascites fluid, clone 4b7, Upstate®

别名:

Anti-NEDLBA, Anti-PP2Ac, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

4b7, monoclonal

物種活性

vertebrates

製造商/商標名

Upstate®

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... PPP2CA(5515)

特異性

未甲基化的PP2A,C亚基

免疫原

对应于人PP2A,C亚基的氨基酸295-309的肽(C-RGEPHVTRRTPDYFL)

應用

抗PP2A抗体,C亚基,去甲基化,克隆4b7,是一种针对PP2A的抗体,可用于IC、IP & WB。
研究子类别
激酶&磷酸酶

神经退行性疾病
研究类别
信号传导

神经科学

品質

已通过免疫印迹法对A431细胞的RIPA裂解液进行了常规评估

標靶描述

36kDa

外觀

0.1M Tris-甘氨酸,pH 7.4,0.15M NaCl,0.05%叠氮化钠,然后添加甘油至30%
腹水

儲存和穩定性

在-20°C下可保存2年

分析報告

对照
阳性抗原对照:货号12-301,未刺激的A431细胞裂解液。 加入2.5µL 2-巯基乙醇/100µL裂解液,煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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Juyeon Hwang et al.
The Journal of biological chemistry, 291(40), 21008-21019 (2016-08-11)
The protein phosphatase 2A (PP2A) subfamily of phosphatases, PP2A, PP4, and PP6, are multifunctional serine/threonine protein phosphatases involved in many cellular processes. Carboxyl methylation of the PP2A catalytic subunit (PP2Ac) C-terminal leucine is regulated by the opposing activities of leucine
Xiaoyu Xia et al.
Cell cycle (Georgetown, Tex.), 14(12), 1938-1947 (2015-04-04)
Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the
Christina Ising et al.
Nature, 575(7784), 669-673 (2019-11-22)
Alzheimer's disease is characterized by the accumulation of amyloid-beta in plaques, aggregation of hyperphosphorylated tau in neurofibrillary tangles and neuroinflammation, together resulting in neurodegeneration and cognitive decline1. The NLRP3 inflammasome assembles inside of microglia on activation, leading to increased cleavage
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C S Moreno et al.
The Journal of biological chemistry, 275(8), 5257-5263 (2000-02-22)
Protein phosphatase 2A (PP2A) is a multifunctional serine/threonine phosphatase that is critical to many cellular processes including development, neuronal signaling, cell cycle regulation, and viral transformation. PP2A has been implicated in Ca(2+)-dependent signaling pathways, but how PP2A is targeted to

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