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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3E4.2, monoclonal
物種活性
human
製造商／商標名
Upstate®
技術
western blot: suitable
輸入
sample type mesenchymal stem cell(s)
同型
IgG1κ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... TBXT(6862)
一般說明
The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.
特異性
Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.
免疫原
Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.
應用
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
Mesenchymal Stem Cells
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.
品質
Routinely evaluated by Western blot on NT2 cell lysates.
Western Blotting:
Recommended working dilutions are 1:1000.
Western Blotting:
Recommended working dilutions are 1:1000.
標靶描述
47 kDa
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
儲存和穩定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Development of antibodies to human embryonic stem cell antigens.
Cai, Jingli, et al.
BMC Developmental Biology, 5, 26-26 (2005)
Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies.
Priddle, Helen, et al.
Stem Cells, 24, 815-824 (2006)
Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomas.
Vujovic, S, et al.
The Journal of Pathology, 209, 157-165 (2006)
T P Yamaguchi et al.
Genes & development, 13(24), 3185-3190 (2000-01-05)
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6
Chromatin remodeling during mouse and human embryonic stem cell differentiation.
Golob, Jonathan L, et al.
Developmental Dynamics, 237, 1389-1398 (2008)
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