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形狀
nanoparticles
包裝
pkg of 1 ea
粒徑
10 nm
λmax
515-520 nm
官能基
maleimide
儲存溫度
−20°C
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一般說明
Gold nanoparticles, 10 nm, maleimide functionalized, conjugation kit is a comprehensive kit that enables easy, one-step conjugation of your thiol-containing ligands to 10 nm gold nanoparticles. The kit includes pre-made mixtures of lyophilized (freeze-dried) maleimide gold nanoparticles, along with all the necessary materials for three independent conjugation reactions. The kit components comprise:
•3 vials of 10 nm Malemide-Activated Gold Nanoparticles (lyophilized)
•Protein Resuspension Buffer - 1.5ml
•Reaction Buffer - 1.5ml
•Quencher Solution - 1.5ml
•Reaction protocol
The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via cysteine residues.
The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation.
The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.
•3 vials of 10 nm Malemide-Activated Gold Nanoparticles (lyophilized)
•Protein Resuspension Buffer - 1.5ml
•Reaction Buffer - 1.5ml
•Quencher Solution - 1.5ml
•Reaction protocol
The gold nanoparticles in this kit are supplied as a lyophilized (freeze-dried) mixture. The conjugation reaction is initiated by simply reconstituting the freeze-dried nanoparticles with the protein of interest, which attaches to the nanoparticle surface via cysteine residues.
The gold conjugation procedure has a quick hands-on time of around 10 minutes, and the conjugate is fully ready for use within 3 hr. Researchers only need to pipette the biomolecule into a vial containing the nanoparticles, quench the reaction, and perform product washing through microcentrifugation.
The provided protocol is optimized for highly efficient covalent conjugation, resulting in stable gold nanoparticle conjugates. Optional protocol steps involve additional materials, such as 10% aqueous BSA (126615) and Tris saline resuspension buffer (PPB002), which are sold separately.
應用
生物相容性，与生物分子的简便结合以及表面等离振子共振产生的独特光学特性使金纳米颗粒可用于各种生物医学研究，例如生物成像，生物传感，光热体温过高，药物递送等。我们的马来酰亚胺功能化金纳米颗粒试剂盒包含即用型混合物，并已针对硫醇修饰的配体（例如寡核苷酸，抗体，抗体片段，蛋白质和肽）的高效性一步偶联进行了优化。
This gold conjugation kit streamlines the process of conjugating your proteins or biomolecules to 10 nm gold nanoparticles. Gold conjugation helps researchers to visualize and assay a specific protein or antibody of interest. Gold conjugates are useful in a wide range of applications like lateral flow testing, immunoblotting, immuno-electron microscopy, immunohistochemical staining, and other bio-assays.
Different sizes of gold nanoparticles perform better in specific applications. For lateral flow and flow cytometry, we recommend using gold nanoparticles with diameters between 20 nm and 100 nm. For immunoblotting or immuno-electron microscopy, the optimal range is 5 nm to 40 nm. For bioassays utilizing dynamic light scattering, gold nanoparticles with diameters ranging from 60 nm to 100 nm are suggested.
Different sizes of gold nanoparticles perform better in specific applications. For lateral flow and flow cytometry, we recommend using gold nanoparticles with diameters between 20 nm and 100 nm. For immunoblotting or immuno-electron microscopy, the optimal range is 5 nm to 40 nm. For bioassays utilizing dynamic light scattering, gold nanoparticles with diameters ranging from 60 nm to 100 nm are suggested.
特點和優勢
•The resulting covalent conjugates offer greater stability compared to those prepared using passive adsorption methods.
•Fast and convenient conjugation reaction with no need for pre-activation.
•A spacer between the gold nanoparticle surface and the conjugated ligand minimizes the impact on the tertiary protein structure. This reduces the likelihood of poor performing conjugates, a common issue encountered with conjugates prepared by passive adsorption.
•Gold surface coating is precisely engineered to minimize non-specific protein binding in biological assays.
•Fast and convenient conjugation reaction with no need for pre-activation.
•A spacer between the gold nanoparticle surface and the conjugated ligand minimizes the impact on the tertiary protein structure. This reduces the likelihood of poor performing conjugates, a common issue encountered with conjugates prepared by passive adsorption.
•Gold surface coating is precisely engineered to minimize non-specific protein binding in biological assays.
法律資訊
CytoDiagnostics 公司产品。
儲存類別代碼
10 - Combustible liquids
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Drs. Fernandes and Baptista discuss gold nanoparticles synthesis and diagnostics regulations, focusing on lateral flow assays.
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