806005

APN-Amine HCl

95%

• 别名: 3-(4-(4-(Aminomethyl)-1H-1,2,3-triazol-1-yl)phenyl)propiolonitrile hydrochloride
• 经验公式（希尔记法）: C₁₂H₁₀N₅Cl
• 分子量: 259.69
• UNSPSC代码: 12352200
• PubChem化学物质编号: 329768982

属性

• 质量水平: 100
• 方案: 95%
• 表单: powder
• 反应适用性: reagent type: cross-linking reagent
• 储存温度: 2-8°C
• SMILES字符串: N#CC#CC1=CC=C(N2C=C(CN)N=N2)C=C1.[H]Cl
• InChI: 1S/C12H9N5.ClH/c13-7-1-2-10-3-5-12(6-4-10)17-9-11(8-14)15-16-17;/h3-6,9H,8,14H2;1H
• InChI key: JZGAUVVBWJHLGN-UHFFFAOYSA-N

说明

应用

APN-Amine is a bifunctional crosslinker for thiol-to-carboxyl coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. The amino group readily reacts with activated carboxylic acids (e.g. NHS-esters) in basic conditions to produce stable amide bonds. The APN group can be used for coupling with free thiols (e.g. labeling of free cysteines in proteins).

其他说明

Standard protein labeling procedure (cysteine labeling):

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid thiol-containing buffers.

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable