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等級
for molecular biology
無菌
0.2 μm filtered
形狀
liquid
成份
Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
技術
microbiological culture: suitable
應用
agriculture
儲存溫度
2-8°C
適合性
nonselective for Escherichia coli
nonselective for coliforms
一般說明
含有分解代谢抑制物的培养基（SOC）是一种富含葡萄糖的微生物生长培养基，主要用于大肠杆菌感受态细胞转化后的复苏。 在这种营养丰富的微生物培养基中培养大肠杆菌细胞，可以抑制克隆载体上携带的lac启动子的意外激活，从而提高含有毒蛋白基因的重组质粒的转化效率。SOC培养基采用低盐配方，含有多肽、氨基酸、水溶性维生素和葡萄糖。
應用
SOC培养基可用于大肠杆菌DH5α 感受态细胞的转化。 它还被用于培养克隆后的白色菌落，以鉴定感兴趣的重组蛋白。
特點和優勢
SOC液体提供：
- 经过滤灭菌的即用型培养基
- 便利的包装规格
- 标准配方
相關產品
产品编号
说明
价格
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Identification of Pathogenicity-Associated Loci in Klebsiella pneumoniae from Hospitalized Patients.
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
商品
General protocols for growth of competent cells and their transformation (uptake of DNA).
感受态细胞生长及其转化（吸收DNA）的通用实验方案。
实验方案
Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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