病毒载体上游工艺流程
优化基因疗法上游生产工作流程，最大限度提高病毒载体滴度，为成功生产奠定基础。然而，由于没有经过验证的流程模板，工艺开发团队必须对病毒载体生产的各个方面进行评估，包括：
- 原料和起始材料
- 细胞培养基
- 细胞和病毒生长、澄清过滤和核酸消化的条件
为了最大限度减少这些工艺投入的变数和风险，基因疗法生产商可以与经验丰富的、在 病毒载体生产 以及相关产品和服务的各个方面都有专长的技术伙伴进行合作。
推荐类别
解冻并扩增细胞和种子
将来自工作细胞库或主细胞库的冷冻细胞解冻并扩增，以增加其数量，为后续处理步骤做准备。
培养基和接种物制备
利用适合细胞生长和病毒生产的营养物质、补充剂和 pH 环境对细胞培养基进行优化。
细胞生长和病毒生产
细胞在生物反应器或其他特定条件下的受控环境中生长，以最大限度地提高病毒产量。
细胞裂解
在 AAV 生产过程中，通过化学或机械方法裂解宿主细胞，使病毒释放到培养基中。
DNA 消化
使用核酸酶消化不需要的全细胞和质粒 DNA。
相关文章
- See how the Sf9 rhabdovirus-free cell line was developed and how we’ve developed a companion chemically-defined insect cell media for protein and viral expression.
- Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.
- A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
- To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
- Read our white paper on the development of a novel cell culture medium for AAV production with multiple HEK293 lineages and process optimization.
- 查看完整内容 (10)
相关文件
- Product Guide: Upstream Chemicals for Bioprocessing
We offer a broad portfolio of raw materials and chemical components suitable for cell culture media formulations and upstream applications used in biopharmaceutical production.
- White Paper: Application of the Sf-Rhabdovirus-Negative (Sf-RVN®) Platform for AAV Production
The Sf-RVN® Platform is a two-part system comprising a proven Sf-9 cell line devoid of Sf-rhabdovirus (the Sf-RVN® cell line) and a chemically defined medium engineered for the cell line, the EX-CELL® CD Insect Cell Medium.
- Brochure: Gene Therapy Capabilities for AAV and Lentivirus Production
Our products and services for AAV, Lentivirus and other viral vectors provide solutions to your most challenging pain points around process development, speed, manufacturing and regulatory guidelines.
- Transcript: Exploring the Potential of Insect Cell Lines for Efficient AAV Production
Interview transcript with GTRI and their findings of using the Sf-RVN® Platform.
相关视频
Benzonase®核酸酶能将 DNA 和 RNA 有效酶解成小于 5 碱基对的片段。
从工艺放大到克服监管障碍，再到快速投产。
从一开始就运用平台工艺，成功放大基因治疗生产规模。
提高基因治疗和疫苗的安全性
相关网络研讨会
用于基因治疗的 AAV 病毒载体的上游生产面临着独特挑战。了解我们的 HEK 平台是如何克服这些挑战的。
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