LLCMK2 (Original)

85062804, rhesus monkey kidney, Epithelial

• UNSPSC Code: 41106514
• biological source: rhesus monkey kidney
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: Epithelial
• products: Not specified
• receptors: Not specified

Properties

• Product Name: LLCMK2 (Original), from rhesus monkey kidney, 85062804, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• biological source: rhesus monkey kidney
• growth mode: Adherent
• karyotype: 2n = 42
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Monkey Rhesus kidney

Cell Line Description

Susceptible to a wide range of viruses including enterovirus, rhinovirus, myxovirus and poxvirus groups.

Application

LLCMK2 (Original) has been used to infect and maintain Trypanosoma cruzi parasite culture[1] and as a medium for Sendai virus titer determination.[2] It has also been used for viral culture.[3][4]

Culture Medium

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 5% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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