S7865
CpGenome Rat Methylated and Unmethylated Genomic DNA Standard Set
Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.24
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General description
The CpGenome Rat Methylated and Unmethylated Genomic DNA Standard Set contains one vial of unmethylated DNA and one vial of methylated DNA generated from the same genomic DNA source.
The CpGenome Rat Unmethylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.
The CpGenome Rat Methylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.
The CpGenome Rat Unmethylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.
The CpGenome Rat Methylated Genomic DNA Standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
Quality
Quantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
Physical form
5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA
Storage and Stability
Upon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Certificates of Analysis (COA)
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