1.04120

Eosin B (bluish) (C.I.45400)

• Synonym(s): Eosin B (bluish) (C.I.45400)
• Empirical Formula (Hill Notation): C₂₀H₆Br₂N₂Na₂O₉
• CAS Number: 548-24-3
• Molecular Weight: 624.06
• MDL number: MFCD00005041
• UNSPSC Code: 41116130

Properties

• form: solid
• Quality Level: 200
• composition: Dye, ≥85% spectrophotometric assay
• pH: 6.2 (25 °C, 10 g/L in H₂O)
• solubility: 300 g/L
• bulk density: 680 kg/m³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: GYYTYUGGVBYJHE-UHFFFAOYSA-L

Description

General description

Eosin B (C.I. 45400) for microscopy Certistain^®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red.
As the dye belongs to the portfolio of Certistain^® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.

Application

for microscopy Certistain^®

Analysis Note

Dye content (gravimetrically): = 85%
Identity

• sodium: passes test
• UV/VIS−Spectrum: passes test

Absorption maximum λ_max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.005 gl; water): 800/1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable