808043

TSKgel^® Phenyl-5PW RP (10 µm) HPLC Columns

L x I.D. 7.5 cm × 4.6 mm, phenyl phase

• Sinónimos: TSKgel^® Reversed Phase HPLC Column (Polymer-based)
• UNSPSC Code: 41115700

Propiedades

• product name: TSKgel^® Phenyl-5PW RP HPLC Column, phase phenyl, L × I.D. 7.5 cm × 4.6 mm, 10 μm particle size
• material: stainless steel
• product line: TSKgel^®
• manufacturer/tradename: Tosoh Phenyl-5PW, 08043
• extent of labeling: 1 meq/mL ligand density (estimate)
• parameter: no limitation salt concentration; 0-100% organic solvent; 1.2 mL/min flow rate; 30 bar max. pressure; 4-60 °C temperature
• technique(s): HPLC: suitable
• L × I.D.: 7.5 cm × 4.6 mm
• matrix: polymer particle platform
• matrix active group: phenyl phase
• particle size: 10 μm
• pore size: 1000 Å pore diameter
• operating pH: 2-12
• separation technique: reversed phase

Descripción

General description

El menor recubrimiento con C18 y fenil en las columnas TSKgel Octadecil-4PW y Fenil-5PW RP resulta en una retención más débil que en las columnas de fase reversa, base de sílice. Estas columnas no son recomendadas para compuestos de bajo peso molecular, pero los poros grandes en estos rellenos (500 Å y 1000 Å respectivamente) las hacen apropiadas para separaciones de péptidos y proteínas. Los poros 1000 Å permiten acceso libre a proteínas y otras biomoléculas con pesos moleculares de hasta 350,000 daltones.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation