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Merck
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Documentos clave

Y1375

Millipore

YPD Broth

suitable for microbiology, NutriSelect® Basic

Sinónimos:

Yeast Peptone Dextrose Broth

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About This Item

Código UNSPSC:
41171607
NACRES:
NA.85

esterilidad

non-sterile

Formulario

powder

composición

Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L

envase

poly bottle of 1 kg
poly bottle of 250 g

fabricante / nombre comercial

NutriSelect® Basic

técnicas

microbiological culture: suitable

color

beige

pH

6.3-6.7

aplicaciones

environmental
food and beverages
microbiology

idoneidad

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

Categorías relacionadas

Descripción general

YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.

Aplicación

YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.

Cantidad

50 g per liter

Nota de preparación

1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Información legal

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000410974
0000361349
0000361349
0000407868
0000343790

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Yeast Extract for use in microbial growth medium

Sigma-Aldrich

Y1625

Yeast Extract

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Biotechnology for biofuels, 5(1), 49-49 (2012-07-21)
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Journal of the American Chemical Society, 136(11), 4333-4342 (2014-03-13)
Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans
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Developmental and comparative immunology, 78, 149-159 (2017-10-08)
The nuclear factor of activated T cells (NFAT) proteins have crucial roles in the development and function of the immune system since they not only regulate activation of T cells but are also involved in the control of thymocyte development
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