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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Sinónimos:

Bradford

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

description

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Quality Level

100

parameter

18-26 °C temperature
50 μL sample volume

storage temp.

2-8°C

General description

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Application

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Biochem/physiol Actions

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Suitability

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principle

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Testing Method

Colorimetric, Endpoint

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • Protein Standard 5 mL

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

flash_point_f

132.8 °F

flash_point_c

56 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Coomassie protein assay reagent

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