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B6916

Supelco

Reactivo Bradford

Bradford Reagent

for 0.1-1.4 mg/ml protein

Sinónimos:

Ensayo de unión de las proteínas al colorante Coomassie, reactivo colorante proteico

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About This Item

Código UNSPSC:
12161500
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nivel de calidad

200

Formulario

solution

temp. de almacenamiento

2-8°C

Descripción general

El análisis de Bradford consiste en añadir azul brillante de Coomassie G-250 a la disolución proteica. El colorante azul Coomassie se asocia con los aminoácidos básicos y aromáticos, causando de este modo un viraje en la absorbancia durante la determinación de proteínas.[1]

Aplicación

El reactivo de Bradford se ha utilizado para determinar la concentración total de proteínas.[2][3][4]

Características y beneficios

  • El reactivo está listo para usar. No se requiere mezcla ni dilución.
  • La aparición del color es rápida. Bastan 5 minutos de incubación y la muestra puede leerse a 595 nm.
  • Los azúcares reductores y las sustancias reductoras junto con los tioles no interfieren con este reactivo.
  • El reactivo es adecuado para microanálisis (1 - 10 μg/ml) y análisis convencionales (50-1400 μg/ml).
  • Puede utilizarse en análisis de placas de micropocillos.
  • Análisis barato.

Otras notas

Ganador del premio CiteAb 2024 como proveedor de éxito en la investigación del Parkinson

Información legal

Aplicación

Referencia del producto
Descripción
Precios

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TP0100

Total Protein Kit, Micro

también adquirido normalmente con este producto

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Pictogramas

Health hazardCorrosion
GHS08,GHS05

Palabra de señalización

Warning

Frases de peligro

H290,H315,H319,H371

Clasificaciones de peligro

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Órganos de actuación

Eyes,Central nervous system

Código de clase de almacenamiento

8B - Non-combustible corrosive hazardous materials

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000261841
0000261841
SLCN3585
SLCK1933
SLCG1879

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Tracey Welham et al.
Journal of experimental botany, 60(12), 3353-3365 (2009-05-29)
Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified
Rosenberg IM
Protein Analysis and Purification: Benchtop Techniques (2006)
Caleigh Mandel-Brehm et al.
Neurology, 93(5), e433-e444 (2019-07-05)
To identify molecular correlates of primary angiitis of the CNS (PACNS) through proteomic analysis of CSF from a biopsy-proven patient cohort. Using mass spectrometry, we quantitatively compared the CSF proteome of patients with biopsy-proven PACNS (n = 8) to CSF
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).
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Protocolos

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Contenido relacionado

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Cuantificación de proteínas

Métodos de cuantificación de proteínas, reactivos y tecnología de inmunoanálisis para la medición precisa de las concentraciones de proteínas en una variedad de muestras.

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Questions

1–10 of 12 Questions  

  1. Talking about the Bradford reagent, I read that: The linear concentration range is 0.1-1.4 mg/mL of protein... does this mean I can prepare these concentrations of protein which will then be diluted when mixed with a certain amount of reagent?

    1 answer

    1. For the Standard 3.1 mL Assay and 96 Well Plate Assay, the protein standards should be prepared in the range of 0.1 - 1.4 mg/mL. This is not the final concentration of the protein after being diluted in the assay.

      For the full protocol, please refer to the document found here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/165/479/b6916bul-ms.pdf

      Helpful?

  2. Does phenol red interfere with the assay? Can I measure protein concentration in cell culture media that has phenol red? It interfered with my BCA assay.

    1 answer

    1. Yes, phenol red at concentrations above 0.5 mg/mL will cause interference. Concentrations below 0.5 mg/mL are expected to be compatible.

      Helpful?

  3. Hallow, Does Bradford Reagent B6916 have color? I don't see it the SDS

    1 answer

    1. The color of this product can range from Faint Yellow-Brown to Light Brown. The exact result will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’: https://www.sigmaaldrich.com/product/sigma/b6916#product-documentation

      Helpful?

  4. Does this product (B6916) suitable for use with buffers with 1% SDS?

    1 answer

    1. A compatibility chart is listed in the bulletin. This chart indicates that this reagent is compatible with concentrations of SDS up to 0.125%.

      Helpful?

  5. Can i use this reagent to quantify protein range from 0-10 ug? May i dilute this bradford reagent, and if i do, will it affect the accuracy of the reading?

    1 answer

    1. The Bradford method has a lower limit of detection of 20 ug/mL. The Lowry method has a lower limit of 10 ug/mL. Concentration of the sample may be necessary.
      See the links below for additional helpful information:

      Protein Quantitation Methods-
      https://www.sigmaaldrich.com/applications/protein-biology/protein-quantitation

      Amicon Centrifugal Filters-
      https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters

      Centricon Centrifugal Filters-
      https://www.sigmaaldrich.com/substance/centriconplus70centrifugalfilter1234598765

      Helpful?

  6. How long is the  reagant stable or can it separate and require mixing prior to use? 

    1 answer

    1. Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat. When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. At somewhat lower temperatures aggregation is also expected to occur, but at relatively slower rates.

      Please see the product data sheet which describes the solution stability of BSA:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/351/531/a8412pis.pdf

      Helpful?

  7. Is the BSA standard supplied with Product B6916, Bradford Reagent?

    1 answer

    1. No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.

      Helpful?

  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?

    1 answer

    1. The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.

      Helpful?

  10. When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?

    1 answer

    1. The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 μg of total protein in 1 mL).

      Helpful?

1–10 of 12 Questions  

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