SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• Sinónimos: Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in insect cells
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: lyophilized powder
• potencia: >0.004 units/mg
• actividad específica: ≥40 units/μg
• envase: pkg of 5 μg
• Nº de acceso UniProt: P10144
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... GZMB(3002)

Descripción

Descripción general

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Acciones bioquímicas o fisiológicas

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Definición de unidad

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

Forma física

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable