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SHPHLIBR

Sigma-Aldrich

MISSION® LentiPlex® Complete Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

Código UNSPSC:
41105904
NACRES:
NA.51

Nivel de calidad

200

Línea del producto

MISSION®

concentración

≥5x108 VP/ml (via p24 assay)

técnicas

capture ELISA: 5 x 108 TU/mL using p24

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma′s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Complete Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 125,000 shRNA constructs from the TRC collection targeting 20,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into sixteen subpools of approximately 6,000-8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

For more information including sample data and uses, please visit our LentiPlex Technical Detail Page.

Otras notas

The MISSION LentiPlex Human Pooled shRNA Libraries consist of Catalog Numbers SHPH01, SHPH15, SHPH2, and SHPHLIBR. Please refer to Table 1 for approximate number of shRNA clone coverage, targeted genes, and subpools for each library.

Amplification and sequencing primers are also provided for downstream hit identification.

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

  • Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
  • Comprehensive, reproducible results from pooled shRNA screens
  • Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Información legal

Lentiviral License Agreement
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Producto relacionado

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Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number

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Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells.
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology

Artículos

Genomic DNA Prep & Deconvolution Services

Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.

Genomic DNA Prep & Deconvolution Services

Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.

Genomic DNA Prep & Deconvolution Services

Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.

Genomic DNA Prep & Deconvolution Services

Deconvolute pooled shRNA samples post-screening to identify significant TRC clones with our service.

Ver todo

Protocolos

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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